fishplot

fishplot visualizes temporal changes in tumor clonal architecture from massively-parallel sequencing-derived subclonal fraction estimates as an R package.

Key Features:

• Visualization: Generates area-style "fish plots" that represent subclonal composition across multiple timepoints.
• Input requirements: Requires simple input data specifying subclonal fractions and sampling timepoints for visualization.
• Dynamic clonal representation: Depicts changes in clonal architecture over multiple timepoints to illustrate tumor heterogeneity, therapeutic response, and resistance dynamics.

Scientific Applications:

• Clonal evolution analysis: Visualizes temporal clonal dynamics to support interpretation of tumor evolution from sequencing data.
• Tumor heterogeneity studies: Aids analysis of intratumoral heterogeneity using sequencing-derived subclonal fraction estimates.
• Therapeutic response and resistance investigation: Supports tracking of clonal expansions or contractions related to therapy over time.

Methodology:

Implemented in R to process input subclonal fraction and timepoint data and generate layered area plots approximating subclonal fractions across timepoints.