flowPloidy

flowPloidy determines DNA ploidy of biological samples by analyzing Flow Cytometry Standard (FCS) files and DNA content histograms.

Key Features:

• Flow Cytometry Data Analysis: Reads Flow Cytometry Standard (FCS) files and extracts flow cytometry data for histogram-based analysis.
• Ploidy Determination: Provides functions to assess DNA ploidy by interpreting DNA content histograms using internal standards.
• Integration with Bioconductor: Operates within the Bioconductor ecosystem on the R platform, enabling interoperability with other Bioconductor packages and workflows.
• Community-Driven Development: Distributed through Bioconductor subject to formal initial review and continuous automated testing to ensure reproducibility and reliability.

Scientific Applications:

• Cancer Research: Enables analysis of tumor DNA content to study tumor biology and progression.
• Aneuploidy and Chromosomal Abnormality Detection: Supports identification of aneuploidies and other chromosomal abnormalities associated with malignancies.
• Experimental Quality Control: Uses internal standards to provide quality control for flow cytometry-based ploidy measurements.

Methodology:

Reads FCS files via the flowCore package (Bioconductor) to extract and analyze flow cytometry data, focusing on DNA content histograms and interpretation against internal standards to determine ploidy.