flowType

flowType performs multidimensional expansion of single-dimensional partitions to phenotype cellular populations from flow cytometry, mass cytometry, and single-cell RT-qPCR datasets.

Key Features:

• Multidimensional partitioning: Expands traditional single-dimensional partitions into multidimensional spaces for finer phenotyping of cell populations.
• High marker support: Handles multiple levels of biomarker expression for up to 96 markers.
• Performance improvement: Latest version reports approximately a 14-fold increase in processing speed (PMID: 24407226).
• R-based BioConductor integration: Implemented within an R-based BioConductor pipeline and integrated with the RchyOptimyx package.
• High-throughput suitability: Facilitates advanced data analysis for high-throughput experiments and large-scale single-cell datasets.

Scientific Applications:

• High-dimensional single-cell assay analysis: Analyzing complex datasets from flow cytometry, mass cytometry, and single-cell RT-qPCR for detailed phenotype characterization.
• Immunology: Precise identification and characterization of immune cell subtypes.
• Oncology: Phenotyping cancer-related cellular populations.
• Developmental biology: Characterizing cell subtypes during development.
• High-throughput experiments: Processing and analysis of large-scale single-cell datasets.

Methodology:

Expands single-dimensional partitions into multidimensional spaces and is implemented within an R-based BioConductor pipeline integrated with the RchyOptimyx package.