Fluid-Squid

Fluid-Squid integrates automated fluidics with quantitative microscopy to support customizable, do-it-yourself multiplexed imaging workflows for characterizing single cells and tissues.

Key Features:

• Multiplexed antibody imaging: Supports oligonucleotide-barcoded antibody panels, including a 36-plex panel for fresh frozen human intestinal tissues and a 39-antibody panel for immune cell characterization.
• Lyophilized panel compatibility: Demonstrates feasibility of lyophilizing multiplexed antibody panels for simplified workflow implementation.
• Sample barcoding and pooling: Enables simultaneous staining of multiple samples to reduce time, costs, and batch effects in single-cell experiments.
• Rapid antibody titration: Facilitates optimization of antibody concentrations through pooled sample processing.
• Automated fluidics: Integrates programmable liquid handling for reproducible, customizable imaging workflows.

Scientific Applications:

• Immune cell population identification: Characterizes immune cell populations and phenotypes from mouse splenocytes and human peripheral blood mononuclear cells.
• Antigen-specific cell detection: Identifies antigen-specific cells within complex immune cell mixtures.
• Tissue imaging: Performs multiplexed imaging of fresh frozen human intestinal tissues for spatial characterization.
• Blood preparation method assessment: Evaluates the impact of various blood preparation methods on cell type retention.

Methodology:

Fluid-Squid combines automated fluidics with microscopy for quantitative, multiplexed imaging using oligonucleotide-barcoded antibody panels.