FlyFactorSurvey

FlyFactorSurvey provides DNA binding specificity data for Drosophila transcription factors to support prediction and analysis of transcription factor–cis-regulatory interactions.

Key Features:

• Extensive data collection: Contains over 400 recognition motifs and position weight matrices derived from more than 200 Drosophila transcription factors, including unpublished motifs.
• Binding specificity representations: Stores recognition motifs, position weight matrices (PWMs), and sequence logos for characterized transcription factors.
• Search and data retrieval: Enables retrieval of binding site information in sequence, matrix, and sequence-logo formats for individual TFs, groups of TFs, or the full set of characterized TFs.
• Motif similarity matching: Identifies motifs in the database that share similarity to a query matrix for comparative motif analysis.
• Genomic distribution visualization: Provides distribution data and visualization of motif occurrences across the Drosophila genome.

Scientific Applications:

• Predictive modeling: Supports prediction of potential TF–target interactions and reconstruction of regulatory relationships.
• Comparative genomics: Facilitates comparison of motifs across transcription factors to assess conservation and divergence of regulatory elements.
• Functional genomics: Links motif occurrence data with genomic locations to infer potential regulatory roles of TFs in developmental and disease-related contexts.

Methodology:

Binding specificities were determined using the bacterial one-hybrid system.