Fly Terminalia Database

Fly Terminalia Database maps transcription factor expression during pupal development of male terminalia in Drosophila melanogaster to provide spatially resolved data for studying gene regulatory networks underlying terminalia morphogenesis and evolution.

Key Features:

• Comprehensive RNA-seq Data: Contains RNA sequencing data from whole male terminalia across pupal stages of Drosophila melanogaster capturing dynamic expression patterns of genes involved in genital and anal structure formation.
• In situ Hybridization Validation: Includes in situ hybridization results for 100 highly expressed transcription factors, providing spatial validation of expression within specific terminalia structures and substructures.
• Patterned Expression Mapping: Reveals patterned expression with distinct transcription factors marking separate anatomical features of the male terminalia during pupal development.

Scientific Applications:

• Gene Regulatory Network Analysis: Enables investigation of transcriptional networks that govern development of Drosophila terminalia.
• Developmental Biology: Supports studies of molecular mechanisms directing formation of specific terminalia structures during pupal development.
• Evolutionary Biology: Facilitates exploration of the genetic basis of rapid morphological evolution in Drosophila terminalia.
• Comparative Genomics: Allows comparison of gene expression patterns across species to identify conserved and divergent regulatory elements.

Methodology:

Performed RNA sequencing on whole male terminalia during pupal development followed by in situ hybridization for a curated set of 100 transcription factors.