FlyTF

FlyTF catalogs and annotates site-specific transcription factors in Drosophila melanogaster by integrating computational predictions and experimental evidence to characterize DNA-binding and regulatory properties.

Key Features:

• Comprehensive repository: Contains 1052 FlyBase identifiers representing putative site-specific transcription factors in Drosophila melanogaster.
• Annotation scope: Provides annotations of DNA-binding characteristics and regulatory functions derived from computational prediction and experimental verification.
• Evidence vocabulary and GO mapping: Uses a defined vocabulary for classifying experimental evidence that is translated into Gene Ontology (GO) annotations in collaboration with FlyBase for incorporation into official GO records.
• Source datasets: Curation is based on existing FlyBase/Gene Ontology annotations and data from the DBD Transcription Factor Database.
• Computational framework: Implemented on the InterMine framework to integrate TF annotations with associated genomic information.

Scientific Applications:

• Transcriptional regulation analysis: Enables analysis of DNA-binding and regulatory properties of transcription factors in Drosophila melanogaster.
• Candidate TF selection: Provides a curated list of 1052 FlyBase identifiers for experimental or computational studies of gene regulation.
• Functional annotation integration: Converts literature-derived experimental evidence into GO annotations to facilitate incorporation into FlyBase GO records.

Methodology:

Integrates computationally predicted and experimentally verified TFs; applies a defined vocabulary for experimental evidence translated into Gene Ontology (GO) annotations in collaboration with FlyBase; curates 1052 FlyBase identifiers using FlyBase/GO annotations and the DBD Transcription Factor Database; stores and links data via the InterMine framework.