frogs_demultiplex

frogs_demultiplex demultiplexes inner-barcoded next-generation DNA sequencing reads to assign reads to individual samples for downstream high-throughput sequencing analyses.

Key Features:

• Sample-Specific Read Splitting: Identifies inner barcodes within reads and assigns reads to their corresponding samples based on those barcodes.
• Integration with Galaxy Project: Integrates with the Galaxy Project to operate within Galaxy workflows and leverage Galaxy's tracking and reproducibility capabilities.

Scientific Applications:

• Genomic Studies: Facilitates analysis of genomic variation across samples by producing sample-specific read sets.
• Transcriptomics: Enables organization of RNA-seq reads into sample-specific datasets for gene expression analysis.
• Metagenomics: Assists in classification and quantification of microbial communities by assigning reads from complex mixtures to their source samples.

Methodology:

Reads sequencing data files, identifies inner barcodes within each read, and assigns reads to corresponding samples based on barcode matches.