FungiProteomeDB

FungiProteomeDB provides molecular weight (MW) and isoelectric point (pI) data for fungal proteins to support proteomic analyses across the fungal kingdom.

Key Features:

• Extensive Data Coverage: Contains protein sequence data from 685 fungal species totaling over 7 million protein sequences.
• Calculated Molecular Weight and Isoelectric Point: Computes and stores precise molecular weight (MW) and isoelectric point (pI) values for each protein sequence.
• Virtual 2D Proteome Map Visualization: Plots proteins by pI and MW to generate virtual 2D proteome maps for cross-species comparison of proteome diversity.
• Proteomics-relevant Metrics: Provides MW and pI parameters applicable to experimental techniques such as 2D gel electrophoresis, liquid chromatography–mass spectrometry (LC-MS), and X-ray crystallography.

Scientific Applications:

• Comparative Proteomics and Evolutionary Analysis: Enables comparison of proteome diversity across fungal species to infer evolutionary relationships and functional adaptations.
• Experimental Proteomics Planning: Informs design and interpretation of 2D gel electrophoresis, LC-MS, and X-ray crystallography experiments by supplying MW and pI data.
• Drug Discovery and Enzyme Engineering: Supports identification and characterization of candidate proteins for drug discovery and enzyme engineering using proteome-wide physicochemical properties.
• Pathogenesis Studies: Aids investigation of fungal pathogenic mechanisms by providing proteomic parameters for candidate virulence factors and related proteins.

Methodology:

Molecular weight (MW) and isoelectric point (pI) values were calculated computationally for the included fungal protein sequences.