gcscreen

Open-source R package for the analysis of kinetic phenotypic screens read as growth curves in 96-well microplates. Implements a benchmarked, simulation-validated pipeline that goes from raw OD600 trajectories to a hit list: plate-level quality control (control CV, Z'-factor, SSMD), within-plate positional correction by median polish (B-score), normalisation to the median of in-plate controls, and hit calling requiring convergence between per-plate ANOVA and a library-wide linear mixed model with Benjamini-Hochberg FDR control. Also extracts kinetic parameters (maximum specific growth rate, maximum OD, lag time) and supports functional principal component analysis and Monte Carlo validation of pipeline operating characteristics.