Gene Array Analyzer

Gene Array Analyzer analyzes CEL files from Affymetrix GeneChip Gene 1.0 ST Arrays to detect alternative splicing events and assess transcript-level expression for splicing studies.

Key Features:

• CEL file processing: Processes CEL files derived from Affymetrix GeneChip Gene 1.0 ST Arrays, which share a design similar to exon arrays used in differential splicing analysis.
• Alternative splicing detection: Identifies alternative splicing events from gene-array data at exon and transcript levels.
• EAA-based algorithms: Builds on the Exon Array Analyzer (EAA) and incorporates its algorithms and processing steps adapted for Gene 1.0 ST arrays.
• Visualization: Generates visualizations and result files summarizing detected splicing events and expression measurements.

Scientific Applications:

• Alternative splicing research: Enables systematic identification and profiling of alternative splicing events from gene-array experiments.
• Developmental biology: Supports profiling of alternative splicing during processes such as embryonic heart development.
• Pathology studies: Facilitates identification of splicing events that are activated under pathological conditions.

Methodology:

Processes CEL files from Affymetrix GeneChip Gene 1.0 ST Arrays, applies algorithms and processing steps derived from the Exon Array Analyzer to detect and visualize alternative splicing events and transcript-level expression.