GENESIS

GENESIS generates Bruker-compatible pulse programs for NOAH (NMR by Ordered Acquisition using 1H detection) supersequences to enable acquisition of multiple 2D NMR datasets within reduced experiment durations.

Key Features:

• Systematic pulse program generation: Automates creation of pulse programs for arbitrary NOAH supersequences, reducing manual effort and minimizing program construction errors.
• Bruker compatibility: Produces pulse programs formatted for direct use on Bruker spectrometers.
• Acquisition and processing scripts: Outputs acquisition and processing scripts required to run and process NOAH experiments.
• Module concatenation and combinatorial exploration: Supports concatenation of five or more modules, enabling exploration of thousands of potential supersequences.
• Integration of methodological advances: Incorporates new developments such as solvent suppression techniques, pure shift NMR modules, and artifact reduction strategies for HMBC and HMQC modules.

Scientific Applications:

• Structural biology and biochemistry: Enables rapid and efficient acquisition of high-quality 2D NMR data for structural characterization and biochemical studies.
• Method development and optimization: Facilitates testing and optimization of NOAH supersequences by exploring large numbers of concatenated module combinations.
• High-throughput multi-2D acquisition: Supports acquisition strategies that obtain multiple 2D NMR datasets within reduced experimental timeframes.

Methodology:

Systematic generation of Bruker-compatible pulse programs with the ability to incorporate new modules such as solvent suppression, pure shift NMR modules, and artifact reduction strategies for HMBC and HMQC.