GenomeCRISPR

GenomeCRISPR provides a curated, queryable repository of CRISPR/Cas9 high-throughput screening results to support gene-to-phenotype mapping and functional genomic analyses.

Key Features:

• Extensive Data Repository: Contains over 550,000 single guide RNAs (sgRNAs) from 84 distinct experiments across 48 human cell lines and aggregates published CRISPR/Cas9 screens performed in human cells.
• Data Mining Tools: Provides capabilities to explore phenotypic outcomes and compare results across screens at the sgRNA and gene level.
• Phenotypic and Genomic Track Views: Includes phenotypic and genomic track representations for detailed examination and comparative analysis of sgRNA performance and screen conditions.
• API Access: Provides an Application Programming Interface (API) for automated data access and batch downloads to support large-scale integration and analysis.

Scientific Applications:

• Systematic Functional Genomics: Supports genome-scale functional analyses via CRISPR/Cas9-mediated gene perturbations.
• Gene-to-Phenotype Discovery: Enables identification and comparison of gene-to-phenotype relationships and candidate genes implicated in disease mechanisms.
• Cross-Screen Comparative Analysis: Facilitates comparison of phenotypic effects of sgRNAs across multiple screens and human cell lines.

Methodology:

Aggregates sgRNA-level screening data from published CRISPR/Cas9 screens in human cells and exposes these data via data mining tools, phenotypic and genomic track views, and an API for programmatic access.