GenomeRNAi

GenomeRNAi compiles and curates RNA interference (RNAi) phenotype and reagent data for human and Drosophila to support loss-of-function functional genomics studies.

Key Features:

• Comprehensive Data Collection: Compiles RNAi phenotype entries with associated reagent information and assessments of reagent efficiency and specificity.
• Data Accessibility: Provides downloadable screen datasets including gene–reagent–phenotype associations and screen metadata.
• Integration Capabilities: Enables in-links via GenomeRNAi screen IDs and external gene or reagent identifiers to support cross-referencing with other resources.
• Visualization Tools: Operates a distributed annotation system (DAS) server to visualize phenotypes and reagents in a genome browser context.
• Identification of Key Genes: Identifies and lists "frequent hitters," genes that exhibit phenotypes across multiple screens.
• Structured Annotation and Submission: Applies structured annotation guidelines and accepts direct researcher submissions via a submission template.

Scientific Applications:

• Functional genomics: Use curated RNAi phenotype data to identify gene functions through loss-of-function screens.
• Human genetics and developmental biology: Support studies in human genetics and Drosophila developmental biology by providing curated phenotype and reagent data.
• Reagent validation and experimental design: Inform selection and assessment of RNAi reagents and aid interpretation of RNAi screen results.

Methodology:

Data are extracted from scientific literature and accepted via researcher submissions; downloadable gene–reagent–phenotype associations and screen metadata are provided; a DAS server enables genome-browser visualization and in-links via GenomeRNAi screen IDs and external gene or reagent identifiers.