GENTANGLE

GENTANGLE enables the computational design of two overlapping genes translated in different reading frames within a microbial genome to add reliable control mechanisms in engineered organisms for increased stability.

Key Features:

• Containerized deployment: The pipeline utilizes Docker/Singularity containers for high-performance execution.
• Arbitrary gene pair input: The software accepts user-specified gene pairs for design and testing of gene entanglements.

Scientific Applications:

• Microbial engineering: The tool designs and tests gene entanglements to improve efficiency of deploying successful designs in genetically engineered systems.
• Genetic stability control: Overlapping gene pairs provide additional control mechanisms to increase stability of engineered organisms.

Methodology:

The pipeline is containerized using Docker/Singularity and operates on arbitrary sets of user-specified gene pairs to design and test gene entanglements.