GEPREP

GEPREP compiles RNA-seq gene expression profiles of exercise responses across multiple species and tissues, providing curated metadata and search and visualization capabilities.

Key Features:

• Comprehensive Data Collection: GEPREP includes 69 RNA-seq datasets of pre- and post-exercise, comprising 26 human datasets (1120 samples) and 43 mouse datasets (1006 samples).
• Curated Metadata: Metadata for each sample were manually curated, including subject information, exercise interventions, sampling sites, and post-processing methods.
• Uniform Data Processing: Data were uniformly processed and batch effects were corrected.
• Multi-Tissue Coverage: Human samples include skeletal muscle (977 samples, 87.2%) and blood (143 samples, 12.8%); mouse samples span 9 tissues with skeletal muscle (359 samples, 35.7%) and brain (280 samples, 27.8%) as the main fractions.
• Data Accessibility: Metadata and gene expression profiles are freely accessible for search, visualization, and download.

Scientific Applications:

• Exercise Physiology Research: GEPREP enables investigation of gene expression changes in response to physical activity across multiple tissues and species.
• Cross-Species Comparative Analysis: The atlas supports comparison of exercise responses between human and mouse models.
• Tissue-Specific Exercise Response Studies: Researchers can examine how different tissues such as skeletal muscle, brain, and blood respond to exercise interventions.

Methodology:

GEPREP was built through comprehensive collection of high-quality RNA-seq data on exercise responses, with manual curation of sample metadata, uniform data processing, and batch effect correction.