GFAP

GFAP annotates plant genes by assigning protein domain, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations using a reference database of protein sequences from 236 plant species across 63 families.

Key Features:

• Extensive Database: Uses protein sequences from 236 plant species across 63 families annotated with Pfam, Gene Ontology (GO), and KEGG.
• Annotation Types: Provides protein domain (Pfam), GO term, and KEGG pathway annotations for input protein sequences.
• High-throughput Performance: Performs rapid database searches capable of retrieving protein domain, GO, and KEGG information for approximately 43,000 genes in about four minutes.
• Functional Modules: Includes sequence alignment, statistical analysis, and graphical representation modules to support annotation workflows.

Scientific Applications:

• Plant Gene Functional Annotation: Assigns functional annotations to plant genes to improve precision in gene function prediction.
• Comparative Reference-based Annotation: Leverages closely related reference species across the database to refine functional annotation accuracy.
• Large-scale Genomics: Supports high-throughput annotation of large gene sets for plant genomics studies.

Methodology:

Maps query protein sequences to a curated protein sequence database from 236 plant species and annotates them by mapping to Pfam, GO, and KEGG; performs sequence alignment, statistical analyses, and graphical output; uses rapid database search operations enabling retrieval of annotations for ~43,000 genes in ~4 minutes.