GIFT

GIFT measures fiber diameters in scanning electron microscopy (SEM) images of electrospun materials to provide quantitative characterization of fiber morphology.

Key Features:

• Implementation: Implemented as an ImageJ macro for automated analysis of SEM images.
• Automated measurement: Automates measurement of fiber diameters from SEM images.
• Distance-frequency analysis: Distinguishes and quantifies fiber diameters by analyzing distance frequency distributions within images.
• Adjustable image-processing parameters: Allows user adjustment of image-processing parameters used during analysis.
• High throughput: Designed for rapid processing of large datasets to enable high-throughput measurement.
• Output options: Produces raw measurement data and average fiber diameter summaries.
• Comparative performance: Reported comparable or lower average error rates, particularly for very small fibers, and reduced processing time per image compared with other tools.

Scientific Applications:

• Material characterization: Provides quantitative fiber diameter distributions for characterization of electrospun materials.
• Quality control: Supports quality control by enabling rapid assessment of fiber diameter consistency.
• Electrospinning optimization: Facilitates optimization of electrospinning process parameters through quantitative diameter measurements.
• Small-fiber analysis: Enables precise measurement of very small fibers where accuracy is critical.

Methodology:

Implemented as an ImageJ macro that measures fiber diameters by analyzing distance frequency distributions within SEM images and accepts adjustable image-processing parameters.