GMWI-webtool

GMWI-webtool computes the Gut Microbiome Wellness Index (GMWI) from stool shotgun metagenomic taxonomic profiles and integrates α-diversity metrics to quantify gut microbiome health based on relative abundances of health-prevalent and health-scarce species.

Key Features:

• Calculation of GMWI: Computes the Gut Microbiome Wellness Index (GMWI) by evaluating relative abundances of health-prevalent and health-scarce species to provide a probabilistic assessment of disease likelihood.
• Data input and processing: Accepts stool shotgun metagenome taxonomic profiles and calculates species relative abundances and α-diversity metrics.
• Visualization and comparison: Generates visualizations and enables comparison against a pooled reference dataset of metagenomes for contextual benchmarking.
• Data export: Exports analysis results as .csv files and allows download of high-resolution figures.

Scientific Applications:

• Case–control microbiome comparison: Quantifies differences in gut microbiome composition between cases and controls using the GMWI metric.
• Microbial marker identification: Supports identification of potential microbial markers associated with health and disease by highlighting health-prevalent and health-scarce taxa.
• Clinical and translational research: Applies to studies in gastroenterology, immunology, and personalized medicine linking microbiome composition to health outcomes.

Methodology:

Analyzes stool shotgun metagenomic taxonomic profiles to quantify species relative abundances and α-diversity, and computes GMWI using an algorithm based on predefined health-prevalent and health-scarce species criteria.