GOblet

GOblet annotates anonymous nucleotide or protein sequences with Gene Ontology (GO) terms by sequence similarity to curated protein databases to support functional annotation across species.

Key Features:

• Database Versatility: Uses curated protein databases specific to humans, murines, invertebrates, plants, and sp-trembl, each with their respective GO mappings.
• Flexible Input Options: Accepts single-sequence and batch submissions for annotation.
• Customizable Analysis Parameters: Allows selection of database sources, alignment thresholds, and sensitivity levels for tailored annotation.
• Comprehensive Result Presentation: Produces survey statistics summarizing major GO categories across submitted sequences and detailed per-sequence GO annotations.

Scientific Applications:

• Genome projects: Functional annotation of gene products in genome-scale sequencing projects using Gene Ontology terms.
• Small sequencing endeavors: Annotation of individual or small sets of anonymous nucleotide or protein sequences.
• Annotation of underrepresented species: Enables GO-based annotation for sequences from species sparsely represented in public databases by leveraging cross-species protein databases.
• Computational classification: Provides structured GO vocabulary outputs suitable for computer-based classification and downstream analyses.

Methodology:

Performs sequence similarity searches against curated protein databases (human, murines, invertebrates, plants, and sp-trembl) using Gene Ontology mappings and user-selected alignment thresholds and sensitivity levels, and was validated on a reference set of over 6,000 human proteins.