GoPeaks

GoPeaks identifies peaks in genome-wide histone modification profiles from CUT&Tag sequencing data to map regulatory elements such as enhancers and promoters.

Key Features:

• Optimized for CUT&Tag histone modifications: Tailored for histone modification profiles generated by CUT&Tag and detects diverse peak profiles associated with histone marks.
• Enhanced sensitivity for H3K27ac: Demonstrates superior sensitivity in identifying H3K27ac peaks compared with standard peak calling algorithms.
• Versatile peak detection: Detects narrow peaks (e.g., H3K4me3 and transcription factors) and accommodates broad marks (e.g., H3K27ac and H3K4me1) with adjustable parameters step, slide, and minwidth.

Scientific Applications:

• Transcriptional Regulation: Mapping histone modifications to investigate how different marks influence gene expression.
• Epigenetic Studies: Profiling epigenetic changes across the genome to study biological processes and disease-associated regulation.

Methodology:

Implements a peak-calling algorithm with adjustable parameters (step, slide, minwidth) and has been rigorously compared against commonly used peak calling methods.