GraBCas

GraBCas predicts potential cleavage sites of caspases 1-9 and granzyme B in protein substrates, focusing on cleavage at the carboxyl terminal of aspartate residues to support analysis of apoptotic protease activity.

Key Features:

• Target proteases: Predicts cleavage sites for caspases 1-9 and granzyme B in protein sequences.
• Primary specificity: Accounts for cleavage at the carboxyl terminal of aspartate residues.
• Score-based prediction: Uses a scoring system to identify potential cleavage sites, including those that deviate from consensus motifs.
• Sequence analysis and fragment estimation: Analyzes protein sequences and estimates sizes of resulting cleavage fragments.
• Validation: Predictions have been tested on known substrates.

Scientific Applications:

• Cleavage-site identification: Identifies candidate cleavage positions in proteins targeted by caspases 1-9 and granzyme B.
• Substrate discovery and validation: Supports exploration and experimental validation of novel protease substrates and uncharacterized cleavage sites.
• Apoptosis mechanism analysis: Aids molecular-level analysis of protease-mediated events in apoptotic pathways by predicting cleavage outcomes and fragment sizes.

Methodology:

Analyzes protein sequences with a score-based prediction system to locate cleavage sites and estimate resulting fragment sizes, with prediction performance evaluated on known substrates.