GTXplorer

GTXplorer analyzes evolutionary relationships among glycosyltransferases that adopt the GT-A fold to connect sequence, structure, and function for studies of carbohydrate biosynthesis.

Key Features:

• Centralized evolutionary dataset: Aggregates evolutionary data derived from primary protein sequences of GT-A fold families into a unified resource.
• Comparative sequence analysis: Enables in silico comparative sequence analyses to inform hypotheses about glycosyltransferase function across clades, families, and subfamilies.
• Phylogenetic tree exploration: Represents evolutionary relationships among GT-A families using phylogenetic trees to identify phylogenetically relevant families for comparison.
• Alignment view with annotated weblogo stacks: Provides residue-level alignments and annotated weblogo stacks specific to selected clades, families, or subfamilies within the GT-A core for sequence-variation analysis.
• Data export for downstream analysis: Exports evolutionary data to support external, downstream computational analyses.

Scientific Applications:

• Function annotation: Supports characterization and functional inference of understudied glycosyltransferases within GT-A fold families.
• Enzyme engineering: Informs design and engineering of novel glycosyltransferases for applications in medicine and biotechnology.
• Synthetic biology: Provides evolutionary and sequence-structure-function insights to guide synthetic biology efforts involving glycosyltransferases.
• Comparative glycomics and evolution: Facilitates comparative glycomics studies to investigate evolutionary trajectories and sequence determinants of carbohydrate biosynthesis.

Methodology:

Integrates multiple data streams into a cohesive format to present evolutionary information for comparative glycomics and hypothesis generation about enzyme function.