H-DBAS

H-DBAS integrates H-Invitational full-length cDNAs with RNA-Seq tag data to catalog human alternative splicing events and annotate their translational potential by correlating 148,376,598 RNA-Seq tags derived from cytoplasmic, nuclear, and polysome fractions with AS exons and splice junctions.

Key Features:

• H-Invitational cDNA integration: Uses H-Invitational full-length cDNAs as the curated reference for alternative splicing exon and junction annotation.
• RNA-Seq tag dataset: Incorporates 148,376,598 RNA-Seq tags derived from RNAs extracted from cytoplasmic, nuclear, and polysome fractions.
• Correlation-based annotation: Enhances annotations by correlating RNA-Seq tag presence with AS exons and splice junctions.
• Translational evidence for exons: Identifies approximately 90,900 exons that are likely to be involved in protein synthesis based on tag distribution.
• Nuclear-specific junctions: Highlights 254 alternative-splicing junctions unique to nuclear RNA transcripts, indicating potential non-translated events.
• Comparative genomics viewer: Provides a comparative genomics viewer to empirically examine evolutionary dynamics of alternative splicing across species.
• Focus on human AS: Specializes in human alternative splicing annotation and analysis.

Scientific Applications:

• Alternative splicing characterization: Catalogs and annotates human AS exons and splice junctions for downstream analyses.
• Translation vs nuclear-retention assessment: Distinguishes AS events with polysome-associated evidence of translation from junctions restricted to nuclear transcripts.
• Exon functional annotation: Supports identification of exons likely contributing to protein synthesis for functional genomics studies.
• Evolutionary analysis of AS: Enables cross-species examination of AS evolutionary dynamics using comparative genomics data.

Methodology:

Comprehensive analysis of 148,376,598 RNA-Seq tags correlated with AS exons and splice junctions derived from H-Invitational full-length cDNAs, combined with comparative genomics examination.