hGPCRnet

hGPCRnet constructs an extended human GPCR network by integrating protein-protein interaction and cell-type-specific protein expression data to delineate GPCR signaling pathways.

Key Features:

• Extended GPCR network: Constructs an interconnected network of human GPCRs, G-protein coupled receptor kinases (GRKs), Gα subunits, β-arrestins, and effectors.
• Protein-protein interaction integration: Incorporates protein-protein interaction (PPI) data involving GPCRs, GRKs, Gα subunits, β-arrestins, and effectors.
• Cell-type protein expression integration: Integrates protein expression data across various cell types to represent component availability in each cell type.
• Cell-type-specific pathway delineation: Delineates distinct GPCR signaling pathways specific to different cell types based on integrated PPI and expression data.
• Network-based signaling mapping: Maps signaling connectivity to reveal how receptor activation outcomes depend on the availability of signaling proteins within a cell.

Scientific Applications:

• Cell-type-specific signaling analysis: Investigates how cell-type-specific protein expression influences GPCR signaling pathways.
• GPCR pharmacology and drug discovery: Supports studies in drug discovery by mapping receptor-associated signaling pathways and signaling outcomes relevant to targeting GPCRs.
• Bioinformatics and molecular biology research: Provides a resource for bioinformatics and molecular biology studies of GPCR-mediated physiological processes.
• Disease mechanism investigation: Facilitates analysis of GPCR signaling alterations and their links to disease mechanisms across different cell types.

Methodology:

Integrates protein-protein interaction data for GPCRs, GRKs, Gα subunits, β-arrestins, and effectors with protein expression data across various cell types to construct an extended human GPCR network and delineate cell-type-specific signaling pathways.