HASP

HASP generates comparative structural models of Influenza A hemagglutinin protein sequences to enable three-dimensional interpretation of phylogenetic, biochemical, and immunological data.

Key Features:

• Rosetta-based comparative modeling: Uses the Rosetta modeling suite to produce comparative models of hemagglutinin amino acid sequences.
• Comprehensive sequence set: Provides models for all publicly available Influenza A hemagglutinin amino acid sequences (8,769 as of the publication).
• High structural fidelity: Models exhibit C-alpha root mean square deviations less than 2 Å when compared to crystallographic templates.
• Sequence alignments: Generates sequence alignments corresponding to modeled hemagglutinin sequences for structural mapping.
• Scalable modeling protocols: Modeling protocols are reported to scale with expanding sequencing efforts.

Scientific Applications:

• Structural interpretation of phylogenetic, biochemical, and immunological data: Enables mapping sequence variation onto three-dimensional hemagglutinin structures to interpret phylogenetic, biochemical, and immunological findings.
• Analysis of mutations affecting pathogenicity and species specificity: Supports analysis of hemagglutinin mutations that influence viral pathogenicity and host specificity.
• Comparative analysis with experimental structures: Facilitates comparison between modeled hemagglutinins and related crystallographic templates.
• Large-scale structural analysis: Supports structural analysis across thousands of Influenza A hemagglutinin sequences to track sequence diversity.

Methodology:

HASP uses the Rosetta modeling suite to generate comparative models for publicly available Influenza A hemagglutinin amino acid sequences, produces corresponding sequence alignments, and evaluates model quality by C-alpha root mean square deviation against crystallographic templates.