HD-eXplosion

HD-eXplosion visualizes hydrogen-deuterium exchange (HDX) mass spectrometry data to generate plots that represent deuterium uptake differences and support analysis of protein dynamics and interactions.

Key Features:

• Chiclet and Volcano Plots: Generates chiclet and volcano plots for bottom-up HDX experiments to depict deuterium uptake differences across conditions.
• Statistical Filtering: Implements statistical filters that allow setting significance thresholds to define and highlight meaningful differences in HDX data.
• Customizable Visualizations: Provides customizable plot options to tailor visual representations of multi-dimensional HDX mass spectrometry datasets for analytical and reporting purposes.

Scientific Applications:

• Proteomics of protein dynamics and interactions: Supports interpretation of HDX mass spectrometry data to characterize protein conformational dynamics and protein–ligand or protein–protein interactions.
• Comparative HDX analysis: Enables comparison of deuterium uptake across experimental conditions to identify regions with significant exchange differences in bottom-up experiments.
• Statistical interpretation of HDX data: Facilitates application of user-defined significance thresholds to increase confidence in detected HDX changes.

Methodology:

The methodology integrates statistical analysis with visualization techniques and uses user-defined significance levels to highlight statistically relevant data points in visual outputs.