Hemolytik

Hemolytik catalogs experimentally determined hemolytic and non-hemolytic peptides and associated annotations to support analysis and design of therapeutic peptides.

Key Features:

• Database content: Approximately 3,000 entries including around 2,000 unique peptides with experimentally determined hemolytic annotations.
• Hemolytic activity data: Hemolytic activities were experimentally evaluated using erythrocytes sourced from up to 17 different origins.
• Data sources: Entries were compiled from published research articles and existing databases including Antimicrobial Peptide Database, Collection of Anti-microbial Peptides, Dragon Antimicrobial Peptide Database, and Swiss-Prot.
• Peptide annotations: Each entry includes peptide name, amino acid sequence, origin, and reported functions.
• Chemical and structural properties: Annotations include chirality, classification as linear or cyclic, and end modifications.
• Structural data: Predicted tertiary structures and assigned secondary structure states are provided for peptides.

Scientific Applications:

• Therapeutic peptide design: Enables selection and optimization of peptide candidates based on hemolytic profiles and annotations.
• Antimicrobial agent development: Supports development of novel antimicrobial peptides by providing experimentally validated hemolytic and functional data.
• Hemolytic risk assessment: Facilitates evaluation of peptide hemolytic potential across erythrocyte origins.
• Structure–function analysis: Supports investigation of peptide secondary and tertiary structures and their membrane interaction-related biophysical properties.

Methodology:

Predicted tertiary structures and assigned secondary structure states were generated for peptide entries.