HisPhosSite

HisPhosSite curates experimentally validated and putative histidine phosphorylation (pHis) proteins and sites to provide a centralized repository for studying pHis across species.

Key Features:

• Extensive Data Collection: Contains 776 verified histidine phosphorylation sites and 2,702 verified pHis proteins across 38 eukaryotic and prokaryotic species, and compiles 15,378 putative pHis sites and 10,816 putative pHis proteins from a dataset covering 1,366 species.
• Curated Annotations: Provides structural and functional annotations for each pHis site and protein to support biological interpretation.
• Advanced Search Capabilities: Implements multiple search engines including motif searches and BLAST searches to locate specific pHis sites or proteins.
• Ortholog Search Integration: Integrates ortholog searches to enable comparative analyses and functional predictions across species.

Scientific Applications:

• Cellular signaling and regulation: Enables investigation of pHis roles in signal transduction, cell cycle regulation, proliferation, differentiation, and apoptosis.
• Disease mechanism studies: Supports research into disease mechanisms where histidine phosphorylation is implicated, including cancer.
• Comparative and evolutionary analysis: Facilitates cross-species comparisons of pHis sites and proteins using ortholog data.
• Site-level proteomics research: Provides a resource for site-specific and systemic studies of histidine phosphorylation in proteomics contexts.

Methodology:

Experimentally validated pHis proteins and sites were curated from published literature, and putative sites and proteins were integrated through ortholog searches.