HnaTyping

HnaTyping performs genotyping of human neutrophil antigens (HNA1–HNA5) from whole genome sequencing (WGS) and next-generation sequencing data by identifying HNA-associated single nucleotide polymorphisms for clinical and research applications.

Key Features:

• Genotyping of HNA Systems: Targets HNA1 to HNA5 and identifies molecular variations across these systems at the genome level.
• Integration with WGS Data: Processes WGS datasets and filters approximately 500 to 3,000 reads per sample to identify SNPs associated with HNA loci, ensuring coverage of SNP loci for HNA1, HNA3, HNA4, and HNA5.
• Multiple Genotyping Techniques: Supports sequence-based typing (SBT), PCR sequence-specific oligonucleotide probes (SSOP), PCR sequence-specific primers (SSP), and PCR restriction fragment length polymorphism (RFLP).
• Parentage Analysis: Assesses parentage possibilities to support genetic studies involving familial relationships.
• Next-Generation Sequencing Compatibility: Leverages next-generation sequencing to enable collective reporting of complete HNA genotyping formats from WGS outputs.

Scientific Applications:

• Transfusion Medicine: Enables detailed analysis of neutrophil antigens to inform transfusion practices and study transfusion-related diseases such as transfusion-related acute lung injury (TRALI).
• Clinical Research on HNA-Associated Conditions: Supports investigation of the molecular basis and clinical roles of HNA systems in disease contexts.
• Genetic and Familial Studies: Facilitates assessment of familial relationships and parentage in genetic research.

Methodology:

Developed using WGS data from six samples obtained from two families within the 1000 Genomes Project, the implementation processes WGS datasets, filters approximately 500–3,000 reads per sample, and identifies SNPs across relevant HNA antigen systems via its algorithmic SNP-identification methodology.