HTP-OligoDesigner

HTP-OligoDesigner automates primer design for high-throughput, sequence-independent gene cloning and site-directed mutagenesis to support production and characterization of proteins for structural and functional studies.

Key Features:

• Automation of Primer Design: Streamlines generation of primers for sequence-independent gene cloning and site-directed mutagenesis across numerous targets.
• Sequence-independent Primer Design: Uses a sequence-independent approach to enable primer design across diverse gene sets without requiring extensive prior sequence knowledge.
• Tm Calculator Integration: Includes an integrated melting temperature (Tm) calculator for rapid assessment of primer thermodynamic properties.

Scientific Applications:

• High-throughput cloning: Provides primers to enable large-scale cloning workflows for many targets in parallel.
• Site-directed mutagenesis: Designs primers for introducing targeted mutations across diverse sequences.
• Protein library generation for structural and functional studies: Supports production of large libraries of soluble proteins for structural analysis, functional characterization, and other biochemical studies.

Methodology:

Employs automated, sequence-independent primer design combined with integrated melting temperature (Tm) calculation to generate primers for gene cloning and site-directed mutagenesis.