HTSvi

HTSvi enables exploratory analysis and visualization of arrayed high-throughput screening (HTS) data to support interpretation of RNA interference (RNAi) and small-molecule perturbation screens.

Key Features:

• Interactive Visualization: Supports visualization of raw and processed HTS measurements for single-channel and multi-channel assays, including image-based screens.
• Quality Control: Provides functionality for assessing dataset quality to detect artifacts and ensure reliable screening results.
• Data Integration: Allows loading and aggregation of per-well raw and analyzed data in a generic tabular format for combined analysis across plates and conditions.

Scientific Applications:

• Drug discovery: Facilitates identification and prioritization of small-molecule hits from high-throughput screening experiments.
• Functional genomics: Supports analysis of RNAi screens to investigate gene function and phenotypic effects at scale.

Methodology:

Leverages statistical techniques within its software framework to process and visualize large-scale HTS datasets across diverse assay types and scales.