HTSvis

HTSvis provides exploratory data analysis and visualization for arrayed high-throughput screening (HTS) experiments to enable quality control, integration, and interpretation of per-well aggregated raw and analyzed data from RNA interference (RNAi), small-molecule, and image-based screens.

Key Features:

• Exploratory visualization: Provides interactive visualization and exploratory analysis of arrayed HTS datasets.
• Quality control checks: Performs quality-control analyses on per-well measurements to assess data quality across plates and assays.
• Single- to multi-channel support: Handles and compares single- and multi-channel measurements, including image-based screening data.
• Per-well aggregated data import: Accepts per-well aggregated raw and analyzed data from diverse assay types and scales in a generic tabular format.
• Integration of screening results: Integrates processed screening results to support interpretation across plates, assays, and perturbation types such as RNAi and small molecules.
• Handling of large HTS datasets: Manages large, arrayed HTS datasets for downstream analysis and interpretation.

Scientific Applications:

• Arrayed high-throughput screening analysis: Analysis and interpretation of arrayed HTS experiments across multiple plates and conditions.
• RNAi perturbation screens: Evaluation and interpretation of per-well results from RNA interference screens.
• Small-molecule/drug screening: Assessment and comparison of screening outcomes from small-molecule perturbation assays.
• Image-based and multiparametric screens: Analysis of multi-channel, image-derived measurements for phenotypic profiling.
• Quality control and exploratory workflows: Routine QC and exploratory data analysis for HTS datasets prior to downstream statistical analysis.

Methodology:

Loads per-well aggregated raw and analyzed data in a generic tabular format, performs quality-control checks, assesses single- and multi-channel (including image-based) measurements, and integrates and visualizes screening results for exploratory analysis and interpretation.