HTT-OMNI

HTT-OMNI integrates and analyzes HTT-interacting proteins (HIPs) and polyQ-dependent omics data to characterize how polyglutamine expansions in huntingtin (HTT) modulate protein interactions and molecular networks relevant to Huntington's disease (HD).

Key Features:

• Integration of Extensive Datasets: Consolidates over 3,000 potential HIPs, primarily from the HINT database, with polyQ-dependent transcriptome and proteome measurements.
• User-Generated Data Integration: Allows incorporation of external user datasets alongside existing HIP and omics data for combined analysis.
• Advanced Filtering Capabilities: Enables filtering of HIPs by experimental metadata such as model organism and tissue to support targeted, tissue-specific analyses.
• Visualization of Molecular Relationships: Visualizes relationships among HTT protein–protein interactions (PPIs), genetic modifiers of HD, and multiomic landscapes.
• Generation and Analysis of Novel Datasets: Supports identification and analysis of previously unreported HTT PPIs and definition of tissue-specific interactions, including comparison of interaction stability across brain regions.

Scientific Applications:

• Modifier Discovery: Facilitates identification and prioritization of proximal HTT interactors and genetic modifiers implicated in HD pathogenesis.
• Tissue-Specific Mechanisms: Enables study of tissue-dependent HTT interaction networks, including cortex- and striatum-specific changes relevant to HD.
• Multiomic Contextualization: Integrates transcriptomic and proteomic data to place HTT PPIs in a multiomic context for hypothesis generation about molecular pathways and targets.

Methodology:

Integrates HIPs (primarily from HINT) with polyQ-dependent transcriptome and proteome measurements, applies metadata-based filtering by organism and tissue, visualizes HTT PPIs and genetic modifiers across multiomic datasets, and supports generation/analysis of novel PPIs and assessment of polyQ effects on interaction stability in cortex and striatum using HD mouse models.