HUGE

HUGE catalogs novel large human cDNAs and curates associated gene and protein data to support functional analysis of KIAA proteins identified by the Kazusa DNA Research Institute.

Key Features:

• Database content: Contains 2,031 KIAA cDNA entries with accompanying gene and protein characteristic tables.
• Manual curation: Includes 451 manually curated KIAA cDNAs prepared as genetic resources for functional studies.
• Functional analyses: Focuses on in-depth functional analysis of proteins encoded by KIAA cDNAs (KIAA proteins).
• HUGEppi: Provides protein–protein interaction data for 84 pairs of KIAA proteins determined by yeast two-hybrid screening.
• ROUGE: Summarizes computer-assisted analyses of approximately 1,000 mouse homologues corresponding to the human large cDNAs identified in the Kazusa project.
• Sequencing origin: Entries derive from extensive human cDNA sequencing efforts performed by the Kazusa DNA Research Institute.

Scientific Applications:

• Functional characterization: Supports elucidation of biochemical and cellular functions of previously unidentified large human proteins (KIAA proteins).
• Interaction mapping: Enables study of protein–protein interaction networks among KIAA proteins using yeast two-hybrid–derived data.
• Comparative genomics: Facilitates evolutionary conservation and functional prediction analyses through ROUGE summaries of ~1,000 mouse homologues.
• Genetic resource provision: Supplies curated KIAA cDNAs as genetic resources for experimental and molecular biology research.

Methodology:

Methods explicitly include extensive human cDNA sequencing, manual curation of KIAA cDNAs, yeast two-hybrid screening for protein–protein interactions, and computer-assisted analyses of mouse homologues.