Human IRES Atlas

Human IRES Atlas maps and annotates putative internal ribosome entry sites (IRESs) across the human transcriptome to support analysis of cap-independent translation initiation and IRES-driven translational regulation.

Key Features:

• Comprehensive database construction: Integrates IRES predictions from IRESfinder, PatSearch, and IRESpy to identify putative IRES elements across the human transcriptome.
• Integration of translation initiation-related features: Incorporates eight distinct genres of translation initiation-related features for each putative IRES.
• Functional evaluation tests: Implements three functional tests—conservation analysis, structural RNA-protein interaction scoring, and conditional translation efficiency assays—to evaluate predicted IRESs.
• IRES-translation initiation interaction maps: Provides detailed interaction maps linking putative IRESs with translation initiation-related features for each gene transcript.
• Hypothesis generation: Integrates diverse data sets and experimental results to enable formulation of testable hypotheses about human IRES-driven translational regulation.

Scientific Applications:

• Study of cap-independent translation: Supports analysis of IRES-mediated cap-independent translation initiation across the human transcriptome.
• Investigation of stress-, disease-, and development-associated regulation: Enables investigation of selective protein synthesis during cellular stress responses, disease states, and developmental processes.
• Prioritization for experimental validation: Aids prioritization of candidate IRES elements for experimental follow-up using conservation, RNA-protein interaction scores, and conditional translation efficiency data.

Methodology:

Integrates predictions from IRESfinder, PatSearch, and IRESpy; collects and integrates eight genres of translation initiation-related data; and applies conservation analysis, structural RNA-protein interaction scoring, and conditional translation efficiency assays as functional evaluation tests.