IBEX

IBEX performs image alignment for multiplexed IBEX (Iterative Bleaching Extends Multiplexity) microscopy to enable high-parameter mapping of cellular phenotypes and multicellular organization in metazoan tissues.

Key Features:

• Multiplexed imaging capacity: Supports imaging of over 65 parameters across diverse tissue types for high-parameter spatial profiling, including applications to the Human Reference Atlas.
• Antibody and fluorophore compatibility: Compatible with more than 250 commercially available antibodies and supports 16 unique fluorophores.
• Iterative staining and bleaching cycles: Uses iterative cycles of antibody labeling, imaging, and chemical bleaching to extend multiplexity.
• Image alignment software: Provides a software solution for aligning images acquired across IBEX imaging cycles.
• Imaging platform compatibility: Operates with standard slides and nonproprietary imaging chambers across various imaging platforms.

Scientific Applications:

• Tissue atlas construction: Enables generation of detailed spatial maps of tissues for reference atlases such as the Human Reference Atlas.
• Cellular phenotype cataloging: Facilitates cataloging diverse cellular phenotypes and multicellular ecosystems within metazoan tissues.
• Disease and therapeutic research: Supports spatially resolved investigation of disease mechanisms and identification of therapeutic targets.
• High-throughput spatial profiling: Enables multiplexed, high-content analysis of cellular and tissue structures in large-scale studies.

Methodology:

Performs computational image alignment of iterative antibody-labeled IBEX image cycles using an image-alignment software solution.