Ibis

Ibis performs base calling for Illumina sequencing, converting fluorescence signals into nucleotide sequences with improved accuracy.

Key Features:

• Accuracy and Speed: Ibis achieves high base-calling accuracy and rapid processing of Illumina sequencing data.
• Increased Output of Usable Reads: By reducing base-calling errors, Ibis increases the proportion of usable reads from millions of short sequencing outputs generated by the Illumina Genome Analyzer.
• Robustness to Sequencing Chemistry and Technology: Ibis maintains performance across different chemistries and Illumina technologies through algorithms that are less reliant on assumptions about the sequencing chemistry and technology.

Scientific Applications:

• Genome assembly: Ibis provides accurate nucleotide sequences from Illumina reads to support genome assembly workflows.
• Variant detection: Improved base calls from Ibis reduce error-induced false positives and false negatives in downstream variant detection.
• Transcriptome analysis: Ibis supplies accurate reads for transcriptome profiling and expression analyses based on Illumina sequencing.
• Metagenomics: Ibis increases usable read yield and base-calling accuracy for metagenomic sequencing studies.

Methodology:

Ibis identifies bases from fluorescence signals generated during Illumina sequencing using advanced algorithms that are less reliant on assumptions about the underlying sequencing chemistry and technology.