ICEKAT

ICEKAT calculates initial rates from continuous enzyme kinetic traces and derives kinetic parameters such as Michaelis-Menten and EC50/IC50 to support high-throughput enzyme kinetics analysis.

Key Features:

• Interactive Kinetic Trace Fitting: Semi-automated, interactive fitting of continuous enzyme kinetic traces with user-defined fitting ranges for initial-rate determination.
• Evaluation of Kinetic Parameters: Derives Michaelis-Menten kinetic parameters and EC50/IC50 values from initial-rate data.
• High-Throughput Screening Integration: Processes large datasets typical of high-throughput screening, demonstrated on over 500 continuous kinetic traces including experimental data for SIRT1 response to small-molecule activators.
• Visualization Capabilities: Generates simultaneous displays of individual initial-rate fits alongside resulting Michaelis-Menten or EC50/IC50 model fits to aid hit identification and assessment of fitting impact.

Scientific Applications:

• Enzyme kinetics parameter estimation: Determination of initial rates and fitting to Michaelis-Menten or EC50/IC50 models for enzymology studies.
• Drug discovery and pharmacology: Evaluation of EC50/IC50 values and enzyme responses to small-molecule activators or inhibitors in drug discovery workflows.
• High-throughput screening analysis: Processing and hit identification from large-scale continuous kinetic datasets.

Methodology:

Semi-automated fitting of continuous enzyme kinetic traces with interactive definition of linear fitting ranges to calculate initial rates and fit Michaelis-Menten or EC50/IC50 models.