iep

iep calculates the isoelectric point (pI) of proteins to determine protein net charge as a function of pH and inform analyses of solubility and interaction properties.


Key Features:

  • Isoelectric Point Calculation: Computes isoelectric point (pI) values for proteins to assess net charge-related properties.
  • Integration with EMBOSS: Implements pI computation within the EMBOSS framework using the EMBOSS C programming libraries.
  • Extensibility via ACD: Supports Application Configuration Definition (ACD) files to modify tool behavior and parameters without changing source code.

Scientific Applications:

  • Protein Analysis: Uses pI values to predict protein solubility and interaction properties relevant to proteomics and structural biology.
  • Bioinformatics Research: Provides pI calculations for incorporation into larger bioinformatics analysis pipelines.

Methodology:

Performs pI computation using EMBOSS C programming libraries with configurable parameters provided via Application Configuration Definition (ACD) files.

Topics

Collections

Details

License:
GPL-3.0
Maturity:
Mature
Cost:
Free of charge
Tool Type:
command-line tool
Operating Systems:
Linux, Windows, Mac
Programming Languages:
C
Added:
11/8/2015
Last Updated:
12/10/2018

Operations

Data Inputs & Outputs

Protein isoelectric point calculation

Publications

Rice P, Longden I, Bleasby A. EMBOSS: The European Molecular Biology Open Software Suite. Trends in Genetics. 2000;16(6):276-277. doi:10.1016/s0168-9525(00)02024-2.

Bleasby AJ, Ison JC, Rice PM. EMBOSS Administrator's Guide. Unknown Journal. 2011. doi:10.1017/cbo9781139151399.

Ison JC, Rice PM, Bleasby AJ. EMBOSS Developer's Guide. Unknown Journal. 2011. doi:10.1017/cbo9781139151405.

Documentation

Downloads

Links