FiberO

FiberO quantifies fiber orientation and organization in biological fibrous tissues through automated image analysis.

Key Features:

• Fiber Orientation Quantification: Performs morphological image openings to quantify fibers orientation.
• Fiber Organization Analysis: Determines and plots fiber continuity in groups to assess organization within tissue.
• Aligned Fiber Continuity Tracking: Tracks the continuity of aligned fibers and quantifies and displays the organized surface(s) in the tissue of interest.
• Performance Benchmarking: Outperforms Directionality, OrientationJ, FiberFit, and CT-FIRE in calculation and plotting of fibers orientation in reference images with known fibers orientation.

Scientific Applications:

• Bone and Tendon Analysis: Evaluates second harmonic generation microscopy images of mouse bones and tendons as examples of biological fibrous tissues.
• Disease Progress Assessment: Assesses alterations in type I collagen molecules and disorganized fiber architecture in disease conditions.
• Therapy Effect Evaluation: Assesses therapy effect on tissue mechanical integrity through quantitative fiber organization metrics.
• Natural and Synthetic Fibrous Tissue Investigation: Investigates fibers orientation and organization across different natural and synthetic fibrous tissues in engineering and science fields.

Methodology:

FiberO performs morphological image openings to quantify fibers orientation, and determines and plots fiber continuity in groups to assess organization.