immunophenotype

immunophenotype performs high-density immunophenotyping of murine genetic knockouts to identify genes and structural correlates underlying immune variation.

Key Features:

• High-density murine platform: Integrates high-density murine immunophenotyping with high-throughput genetic screening.
• High-throughput phenotyping: Performs systematic, high-throughput phenotyping across mouse gene knockouts.
• Monogenic hit discovery: Identified 140 monogenic 'hits' from phenotyping 530 unique mouse gene knockouts.
• Human loss-of-function enrichment: The identified genes are enriched for human genes with poor tolerance to loss of function.
• Dense correlation networks: Reveals dense correlation networks linking immune parameters with each other and with physiological traits.
• Immunologic structures: Defines genetically regulated immunologic structures that constrain individual parameter variability and relate to immunocompetence.

Scientific Applications:

• Genetic resource expansion: Provides an expanded resource of genes affecting immunity derived from murine knockout phenotyping.
• Structural insights: Identifies dense correlation networks and genetically regulated immunologic structures that constrain immune parameter variability.
• Immunocompetence correlation: Enables assessment of immune health by associating the integrity of immunologic structures with overall immunocompetence.

Methodology:

Uses high-throughput phenotyping to systematically evaluate mouse gene knockouts and map complex interaction networks linking immune parameters and physiological traits.