iMRM

iMRM identifies five major RNA modifications—N6-methyladenosine (m6A), 5-methylcytosine (m5C), N1-methyladenosine (m1A), pseudouridine (ψ), and adenosine-to-inosine (A-to-I) editing—simultaneously across Homo sapiens, Mus musculus, and Saccharomyces cerevisiae for transcriptome-wide analysis.

Key Features:

• Simultaneous identification: Identifies m6A, m5C, m1A, pseudouridine (ψ), and A-to-I editing concurrently across Homo sapiens, Mus musculus, and Saccharomyces cerevisiae.
• Feature selection and evaluation: Employs feature selection to optimize prediction and was benchmarked using 10-fold cross-validation and jackknife tests, demonstrating improved performance over existing methods.

Scientific Applications:

• Transcriptome profiling of RNA modifications: Enables analysis of the distribution of m6A, m5C, m1A, ψ, and A-to-I modifications within transcriptomes.
• Functional and disease studies: Supports investigation of the roles of RNA modifications in gene regulation, epigenetics, and disease mechanisms.

Methodology:

Integrates computational prediction techniques with feature selection strategies and evaluates performance using 10-fold cross-validation and jackknife tests.