IndPenSim

IndPenSim simulates industrial-scale fed-batch fermentation for penicillin production using a mechanistic model validated against historical data from a 100,000-liter Penicillium chrysogenum bioreactor.

Key Features:

• Mechanistic Modeling: A structured first-principles mechanistic model adapted and extended to capture key dynamics of penicillin fermentation.
• Environmental Effects Integration: Incorporates dissolved oxygen, viscosity, temperature, pH, and dissolved carbon dioxide effects on the process.
• Nutrient Impact Analysis: Accounts for the influence of nitrogen and phenylacetic acid concentrations on biomass growth and penicillin production rates.
• Data Validation: Predictions are compared with online and offline measurements from real industrial fermentation batches.
• Off-Gas Analysis: Provides detailed simulations of off-gas composition and dynamics aligned with recorded batch data.

Scientific Applications:

• Process systems benchmarking: Provides a mechanistic benchmark for process systems analysis and control studies of penicillin fermentation.
• Control strategy evaluation: Enables evaluation and enhancement of control strategies using simulated responses to manipulated variables.
• Process optimization: Supports studies of process optimization and control system improvements for fed-batch penicillin production.
• Model validation and experimental comparison: Facilitates validation of model outputs against historical industrial batch records and online/offline measurements.

Methodology:

IndPenSim applies manipulated variables from actual fermentation batches as inputs to a structured mechanistic model to generate simulated outputs that are validated against historical batch records from a 100,000-liter Penicillium chrysogenum bioreactor.