INSPEcT-GUI

INSPEcT-GUI analyzes synthesis, processing, and degradation kinetics from transcriptomic data to infer kinetic rates and characterize their effects on premature and mature RNA species.

Key Features:

• Visualization of RNA dynamics: Visualizes gene-level dynamics of premature and mature RNA species to inspect temporal changes in abundance and kinetic rates.
• Model refinement: Refines model fits to experimental data to improve estimation of synthesis, processing, and degradation parameters.
• Regulatory model testing: Tests alternative regulatory models to evaluate mechanisms by which kinetic rates influence gene expression programs.
• Data-independent kinetic exploration: Explores combined effects of synthesis, processing, and degradation rates independently from specific datasets.

Scientific Applications:

• Time-course and steady-state analysis: Applies to both time-course experiments and steady-state conditions to study kinetic changes over time or at equilibrium.
• Analyses with or without nascent RNA data: Supports inference of kinetic rates using datasets that include nascent RNA measurements or only mature RNA.
• RNA-seq–based investigation of gene expression programs: Uses RNA-seq data to investigate how kinetic rates shape gene expression responses and programs.

Methodology:

Inference of synthesis, processing, and degradation rates from transcriptomic (RNA-seq) data.