InteroPORC

InteroPORC predicts protein-protein interaction (PPI) networks across sequenced genomes using Integr8 and interolog-based transfer to elucidate protein relationships across species.

Key Features:

• Integration of Known Interactions and Orthologous Clusters: Combines known PPIs with clusters of orthologous proteins to improve prediction of interactions across species.
• Systematic Transfer via Interologs: Uses interologs (homologous protein pairs) to systematically transfer experimentally observed interactions from model organisms to other species.
• Automated Prediction Across Sequenced Genomes: Applies PPI prediction across all sequenced genomes contained in the Integr8 database.
• Application to Synechocystis: Generated an inferred interaction network for Synechocystis comprising 8783 predicted interactions.

Scientific Applications:

• Systems Biology: Enables reconstruction of organism-level protein interaction networks for systems-level analyses.
• Functional Genomics: Supports functional annotation and prediction of protein roles based on interaction context.
• Therapeutic Target Identification: Aids identification of candidate proteins and interaction partners as potential therapeutic targets.
• Evolutionary Studies: Facilitates comparative analyses to assess conservation and divergence of protein interactions across species.
• Pathway and Network Analysis: Assists analysis of metabolic pathways, signal transduction, and gene regulation through inferred PPIs.

Methodology:

InteroPORC integrates known interactions with clusters of orthologous proteins from the Integr8 database and employs interolog-based transfer to predict PPIs across sequenced genomes, exemplified by an 8783-interaction network for Synechocystis.