i2i

i2i processes mass spectrometry imaging (MSI) data acquired with continuous ionization techniques to generate ion images that account for automatic gain control (AGC)-modulated duty cycles.

Key Features:

• Data Handling Flexibility: Supports both quantitative and nontargeted processing of MSI datasets.
• AGC-aware Pixel Determination: Accounts for automatic gain control (AGC) influence on the mass spectrometer duty cycle when determining pixel dimensions.
• Scan Filter Integration: Integrates one or several scan filters and facilitates interlacing of MS^n and SIM scan datasets.
• High-Resolution Support: Accommodates high-resolution MSI data.
• Rapid Processing: Processes complex datasets in minutes for high-throughput analysis.

Scientific Applications:

• High-resolution spatial molecular imaging: Generates spatial distributions of molecules from continuous ionization MSI data for detailed imaging analyses.
• Proteomics: Supports spatial proteomic investigations using MSI data from continuous ionization sources.
• Metabolomics: Enables spatial metabolomic analyses by converting MSI line-scan data into ion images.
• Pharmaceutical research: Facilitates mapping of drug and metabolite distributions in tissues using continuous ionization MSI with AGC-aware processing.

Methodology:

Implemented in MATLAB, providing computational routines to generate, process, and export MSI data from full scans and complex scan functions, integrate scan filters to interlace MS^n and SIM scans, and account for AGC-modulated duty cycles when determining pixel dimensions.