ISCompare

ISCompare identifies differentially located insertion sequences (DLIS) between related bacterial strains to detect insertion sequence (IS) mobilization and assess its contribution to genome evolution, adaptability, and phenotypic variation.

Key Features:

• Simultaneous Analysis: Profiles multiple IS mobilization events concurrently across related bacterial strains.
• Versatile Input Compatibility: Accepts both complete and draft genome assemblies.
• Efficient Identification Output: Generates a list of candidate DLIS from comparative genome analyses.

Scientific Applications:

• Genome Evolution Studies: Identifies relocated ISs to study their contribution to bacterial genome plasticity.
• Adaptability Research: Detects IS mobilization associated with phenotypic variation and bacterial adaptability.
• Comparative Genomics: Compares related strains to reveal IS-mediated genetic diversity within species.

Methodology:

Implemented in Python 3 and validated by testing on artificial genomes with simulated random IS insertions and on real sequence datasets.