IsoPairFinder

IsoPairFinder identifies pathway intermediates by analyzing paired unlabeled (12C) and isotope-labeled (13C) metabolomics data from gene-edited microbes for biochemical pathway discovery using stable isotope tracing metabolomics.

Key Features:

• Paired Isotope Analysis: Analyzes paired unlabeled (12C) and isotope-labeled (13C) metabolomics data from gene-edited microbes.
• Substrate-Specific Feature Pair Prioritization: Prioritizes substrate-specific feature pairs to efficiently identify biologically relevant intermediates.
• GNPS2 Integration: Integrated into the GNPS2 ecosystem as a web-based workflow.

Scientific Applications:

• Biochemical Pathway Discovery: Identifies pathway intermediates to accelerate novel pathway discovery and validation in gene perturbation experiments.
• Functional Annotation of Microbial Genes: Addresses gaps in metabolic pathway knowledge by integrating genetic manipulation with stable isotope tracing metabolomics.

Methodology:

IsoPairFinder prioritizes substrate-specific feature pairs from paired unlabeled and isotope-labeled metabolomics data to identify pathway intermediates.