ITSoneDB

ITSoneDB provides an extensive, well-annotated reference database of fungal ribosomal RNA Internal Transcribed Spacer 1 (ITS1) sequences to support accurate phylogenetic classification of amplicon-based metagenomic surveys of fungal communities.

Key Features:

• Targeted Sequencing Support: Focuses on ITS1 sequences, which are variable among fungal taxa and flanked by conserved regions suitable for PCR amplification and sequencing on next-generation platforms.
• Comprehensive Annotation: Contains well-annotated ITS1 entries that have been phylogenetically classified to serve as high-quality reference data for metagenomic analyses.
• Facilitation of Accurate Taxonomic Assignment: Provides a curated ITS1 collection to improve assignment of amplicon sequence reads to correct phylogenetic ranks in fungal metagenomics.
• Complementarity to 16S ribosomal DNA resources: Offers ITS1 reference data intended to complement existing 16S ribosomal DNA databases for microbial community studies.
• Enhanced Metagenomic Research Applications: Supplies reference sequences to support analyses of environmental fungal diversity and community composition in amplicon-based studies.

Scientific Applications:

• Environmental fungal community profiling: Enables amplicon-based metagenomic surveys of environmental fungal communities for composition and diversity analyses.
• Taxonomic and phylogenetic classification: Assists taxonomic assignment and phylogenetic placement of fungal ITS1 amplicons in ecological and microbiological studies.
• Ecology, environmental science and microbiology research: Supports studies focused on fungal community composition, dynamics, and ecological interactions.

Methodology:

Careful curation and phylogenetic classification of ITS1 sequences were performed.